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PCTAIRE 1 Antibody

Find CDK16 Antibodies with a specific conjugate such as Biotin, APC, FITC. The conjugates listed below are among those available. Over 10 years' experience for 6000+ recombinant proteins production. Phosphorylation of PAK1 by p35/CDK5 is likely to be implicated in the dynamics of the actin cytoskeleton reorganization. PCTK3 may also phosphorylate substrate proteins, such as PAK1, and control actin dynamics.
Propylene glycol also can be used as a solvent to minimize evaporation. If the solvent evaporates, the coelenterazine will still be present in the vial, so note the volume in PCTAIRE Antibodies the vial prior to storage so that you can adjust the solvent volume to correct for evaporation if needed. Prepare working solutions in aqueous buffers immediately before use.

Avantor® can help equip your life sciences lab with the products, equipment, and supplies you need – whether you work in cell biology, genomics, proteomics, or other fields. ELISA Protocol Troubleshooting and FAQs Antibody FAQs Storage Upon receipt, store at -20°C or -80°C. Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.
AntiFix™ Universal Antigen Retrieval Buffer provides excellent heat unmasking for a wide variety of targets at neutral pH, reducing the need for buffer optimization. A revolutionary antifade mounting medium optimally formulated for preserving fluorescence of our CF® dyes and other fluorochromes. This unique reagent quenches lipofuscin autofluorescence in tissue sections for immunofluorescence staining and can also help to reduce autofluorescence from other sources like extracellular matrix. Some products are shipped with blue ice packs as an extra precaution against high temperatures.

In the presence of cyclin A2, PCTK3 activity was comparable to CDK2 activity. We also found that PCTK3 knockdown in HEK293T cells induced polymerized actin accumulation in peripheral areas and cofilin phosphorylation. Taken together, our results provide the first evidence for the mechanisms of catalytic activation of PCTK3 by cyclin A2 and PKA and a physiological function of PCTK3.
Immunoprecipitated proteins were then detected by immunoblotting using anti-Sec23Ap or anti-PCTAIRE-1/3 antibodies . The crystal structures of monomeric CDK16 represent the first structures from the PCTAIRE family of CDK kinases . The kinase domain exhibits the classical bilobal architecture intermixed with short insertions that help to define the CDK family fold. The complex with indirubin E804 displays some disorder in the kinase N-lobe, consistent with its requirement for a cyclin partner.

The amount of protein transported to the plasma membrane was then measured 60 minutes after temperature shift using immunofluorescence with an antibody directed against the extracellular domain of ts045-G-YFP. The proportion of ts045-G-YFP transported to the plasma membrane in cells expressing GFP, GFP-PCTAIRE-1 or GFP-PCTAIRE-1 was determined (Fig. 6). An 18% reduction in transport was seen in cells coexpressing the kinase-dead mutant. In contrast, cells expressing an active S153A mutant of PCTAIRE-1 showed an equivalent enhanced amount of transport of ts045-G-YFP.
The results of the PDX model further verified the anti-tumor effect of targeting CDK16 in TNBC, which significantly delayed tumor occurrence (Fig. 3F), suppressed tumor growth (Fig. 3G), and finally inhibited tumor progression (Fig. 3H, I). Fresh tumor fragments from breast cancer patients were subcutaneously transplanted into 4-week female SCID/Beige mi

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